What is EN ISO 7899-2:2010+A1:2015?

EN ISO 7899-2:2010+A1:2015 is a technical standard that provides guidelines for the enumeration of intestinal enterococci bacteria in various samples, including water and sediment. The standard was first published in 2010 and later revised in 2015 with an amendment (A1). It forms part of a series of standards developed by the International Organization for Standardization (ISO) and the European Committee for Standardization (CEN) to ensure quality and reliability in microbiological analysis.

The Importance of EN ISO 7899-2:2010+A1:2015

This standard plays a crucial role in monitoring and assessing the microbial quality of water and sediments. Enterococci are indicator organisms that indicate fecal pollution and the potential presence of pathogens, such as E. coli and other harmful bacteria. By following the procedures outlined in EN ISO 7899-2:2010+A1:2015, laboratories can accurately quantify enterococci levels, enabling authorities to make informed decisions regarding the safety of water for various purposes, such as drinking, recreational activities, and environmental management.

Enumeration Technique

The enumeration technique described in EN ISO 7899-2:2010+A1:2015 involves culturing samples on selective agar media, followed by incubation under specific conditions. The standard provides detailed instructions on sample collection, preparation, and plating techniques to ensure reliable and consistent results. Additionally, it specifies the incubation time and temperature required for accurate enumeration, along with criteria for interpreting the results.

Limitations and Considerations

While EN ISO 7899-2:2010+A1:2015 is a valuable standard for assessing microbiological quality, it is important to note its limitations. Enterococci are not specific pathogens themselves but serve as indicators of potential contamination. Other pathogens, such as viruses and parasites, may not be detected through this standard. Furthermore, it is essential to consider regional or national regulations when interpreting results, as different countries may have their own guidelines and permissible limits.

In conclusion, EN ISO 7899-2:2010+A1:2015 is an important technical standard that provides guidelines for the enumeration of intestinal enterococci bacteria in water and sediment samples. By adhering to this standard, laboratories can ensure accurate and consistent results, facilitating informed decisions regarding water safety. However, it is essential to keep in mind the limitations of this standard and consider other potential pathogens that may not be detected through this method.